The agitation by the gujjar community, led by Kirori Singh Bainsla, entered the second day on Tuesday disrupting road and rail traffic in Bharatpur district of Rajasthan.
The authorities have diverted several trains and stopped buses on two routes, official sources said.
Janshatabdi express and Jamunabridge express have been cancelled while movement of two dozen other trains has been affected in the Bharatpur-Sawaimadhopur section, they said.
A Rajasthan State Road Transport Corporation official said that buses on the Hindaun-Bayana and Hindaun-Bharatpur routes have been stopped due to the agitation.
Security forces have been deployed in Pilukapur area as the agitators are camping at the Delhi-Mumbai rail tracks near the region, a senior police official said.
"Additional forces of reserved armed constabulary, railway protection force and government railway police have been deployed for the security of railway stations, rail tracks and nearby areas," the official said.
The gujjars led by Bainsla started an agitation on Monday in Bharatpur district to press for their demand for reservation for the community, even as the Gehlot government stated that it would try to solve the issue through talks.